Abstract
Background aims
To facilitate artificial bone construct integration into a patient's body, scaffolds
are enriched with different biologically active molecules. Among various scaffold
decoration techniques, coating surfaces with cell-derived extracellular matrix (ECM)
is a rapidly growing field of research. In this study, for the first time, this technology
was applied using primary dental pulp stem cells (DPSCs) and tested for use in artificial
bone tissue construction.
Methods
Rat DPSCs were grown on three-dimensional-printed porous polylactic acid scaffolds
for 7 days. After the predetermined time, samples were decellularized, and the remaining
ECM detailed proteomic analysis was performed. Further, DPSC-secreated ECM impact
to mesenchymal stromal cells (MSC) behaviour as well as its role in osteoregeneration
induction were analysed.
Results
It was identified that DPSC-specific ECM protein network ornamenting surface-enhanced
MSC attachment, migration and proliferation and even promoted spontaneous stem cell
osteogenesis. This protein network also demonstrated angiogenic properties and did
not stimulate MSCs to secrete molecules associated with scaffold rejection. With regard
to bone defects, DPSC-derived ECM recruited endogenous stem cells, initiating the
bone self-healing process. Thus, the DPSC-secreted ECM network was able to significantly
enhance artificial bone construct integration and induce successful tissue regeneration.
Conclusions
DPSC-derived ECM can be a perfect tool for decoration of various biomaterials in the
context of bone tissue engineering.
Graphical Abstract
Graphical Abstract
KeyWords
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Article info
Publication history
Published online: March 15, 2022
Accepted:
February 5,
2022
Received in revised form:
November 22,
2021
Received:
June 9,
2021
Identification
Copyright
© 2022 International Society for Cell & Gene Therapy. Published by Elsevier Inc. All rights reserved.
